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). While in the absolutely free ChVLig-AMP, the OB domain is reflected away from the NTase area to completely expose the DNA-binding area higher than the AMP-binding pocket. The peptide section which is destined to become the latch is disordered during the absolutely free ligase and sensitive to proteolysis, but this section is shielded from proteolysis when ChVLig binds to nicked DNA.
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) so which the concave surface of the OB β-barrel matches into your DNA slight groove. This transition การเดิมพันกีฬาออนไลน์ elicits a 63-Å movement of your OB area and spots the latch deep in the DNA key groove.